FIGURE 4.

Down-regulation of extracellular genomic RNA by curcumin. A, HSAECs were pre- and post-treated with curcumin, and supernatants were analyzed for viral genomic RNA copies by qRT-PCR in comparison with untreated and DMSO-treated, MP-12-infected cells. Viral genomic copy numbers in the context of inhibition by synthetic curcumin or dimethoxycurcumin were determined. Supernatants were obtained from infected, inhibitor-treated cells at 24 h post-infection and analyzed by qRT-PCR. B, HSAECs were either pretreated (2 h) or post-treated (3 h) with curcumin, and the viral genomic copy number in the supernatant was determined by qRT-PCR.