FIGURE 6.

The negative effect of insulin on hGH RNA levels is trichostatin A-sensitive. Primary pituitary cells were treated with HDACs inhibitors, trichostatin A (300 nm), nicotinamide (15 mm), or vehicle 1 h before insulin (1, 5, and 15 nm) treatment. Human GH RNA was assessed by qPCR 24 h post-insulin treatment. Trichostatin A but not nicotinamide muted the insulin effect on hGH RNA levels and was significant with 5 and 15 nm insulin. Results were analyzed and are expressed as relative mean change as described in Fig. 4.