FIGURE 4.

Time course of the association of β-satellite DNA with nucleoprotein complexes isolated by immunoprecipition of S phase Mcm3, Pol α, δ, and ϵ. The association was studied by qPCR with primers specific for the 24-bp β-satellite repeat as indicated under “Experimental Procedures.” This repeat has previously been suggested to be replicated in late S phase in human cells (39). The cells were synchronized by double thymidine block to early S phase (0 h) and released to proceed in S phase (2, 4, and 6 h).