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. 2012 Aug 10;287(40):33327–33338. doi: 10.1074/jbc.M112.357996

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Chromatin and matrix association of replicative DNA polymerases during S phase. T98G cells synchronized by serum stimulation were subjected to subnuclear fractionation and relevant proteins were analyzed by Western blotting. A, analysis of marker proteins and selected replication factors in fractionated, asynchronous T98G cells. T, total cell extract; S, soluble fraction; B, chromatin bound fraction; and M, matrix fraction. B, cell cycle progression of the cell synchronization of T98G cells was employed for fractionation. Propidium iodide-stained cells at the indicated times after serum stimulation were analyzed by flow cytometry. C, time course for the occurrence of Pol δ, Pol ϵ, and Cdc45 in fractions from serum-stimulated synchronous T98G cells. Times after the addition of serum are presented on the top of the panel.