TABLE 2.
Plasmids used in this work
| Plasmid | Relevant characteristics | Source or Ref |
|---|---|---|
| pET-15b | E. coli expression vector with N-terminal His6 tag fusion | Novagen |
| pGH313 | PAH1 coding sequence inserted into pET-15b | 9 |
| pGH313-1–752 | PAH1 (1–752 truncation) derivative of pGH313 | This study |
| pGH313-1–646 | PAH1 (1–646 truncation) derivative of pGH313 | This study |
| pGH313-235–752 | PAH1 (235–752 truncation) derivative of pGH313 | This study |
| pGH313-18–862 | PAH1 (18–862 truncation) derivative of pGH313 | This study |
| pGH313-S10A | PAH1 (S10A) derivative of pGH313 | This study |
| pGH313-S677A | PAH1 (S677A) derivative of pGH313 | This study |
| pGH313-S773A | PAH1 (S773A) derivative of pGH313 | This study |
| pGH313-S774A | PAH1 (S774A) derivative of pGH313 | This study |
| pGH313-S773A/S774A | PAH1 (S773A/S774A) derivative of pGH313 | This study |
| pGH313-S788A | PAH1 (S788A) derivative of pGH313 | This study |
| pGH313-5A | PAH1 (S10A/S677A/S773A/S774A/S788A) derivative of pGH313 | This study |
| pRS415 | Low copy E. coli/yeast shuttle vector with LEU2 | 85 |
| pGH315 | PAH1 inserted into pRS415 | 29 |
| pGH315-S10A | PAH1 (S10A) derivative of pGH315 | This study |
| pGH315-S10D | PAH1 (S10D) derivative of pGH315 | This study |
| pGH315-S677A | PAH1 (S677A) derivative of pGH315 | This study |
| pGH315-S677D | PAH1 (S677D) derivative of pGH315 | This study |
| pGH315-S773A | PAH1 (S773A) derivative of pGH315 | This study |
| pGH315-S773D | PAH1 (S773D) derivative of pGH315 | This study |
| pGH315-S774A | PAH1 (S774A) derivative of pGH315 | This study |
| pGH315-S774D | PAH1 (S774D) derivative of pGH315 | This study |
| pGH315-S788A | PAH1 (S788A) derivative of pGH315 | This study |
| pGH315-S788D | PAH1 (S788D) derivative of pGH315 | This study |
| pGH315-4A | PAH1 (S677A/S773A/S774A/S788A) derivative of pGH315 | This study |
| pGH315-4D | PAH1 (S677D/S773D/S774D/S788D) derivative of pGH315 | This study |
| pGH315-5A | PAH1 (S10A/S677A/S773A/S774A/S788A) derivative of pGH315 | This study |
| pGH315-5D | PAH1 (S10D/S677D/S773D/S774D/S788D) derivative of pGH315 | This study |
| pGH315-7A | PAH1 (S110A/S114A/S168A/S602A/T723A/S744A/S748A) derivative of pGH315 | 29 |
| pGH315-S10A-7A | PAH1 (S10A/S110A/S114A/S168A/S602A/T723A/S744A/S748A) derivative of pGH315 | This study |
| pGH315-S10D-7A | PAH1 (S10D/S110A/S114A/S168A/S602A/T723A/S744A/S748A) derivative of pGH315 | This study |