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. 2012 Aug 6;287(40):33412–33423. doi: 10.1074/jbc.M112.402503

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Constitutive DNA damage response in TR and DF cells. A, diagram of the integration site in c-myc:Pu-Py cell lines DF, DR, TF, and TR. B and C, whole cell extracts were isolated after treatment of cells with caffeine (+) for the indicated times (hours) or parallel untreated cultures (−) and immunoblotted for Chk1 and phospho-Chk1^Ser-345 (p-Chk1^ser345). LC, loading control. D and E, whole cell extracts were isolated after overnight treatment with aphidicolin and released into fresh control medium or medium containing caffeine for the indicated time and immunoblotted. F, flow cytometry profiles of asynchronous cultures of c-myc:Pu-Py cell lines.