FIGURE 2.

FOXO1 is expressed in adult mouse gonadotrope cells. A and B, dual label IF was performed on paraffin-embedded sections of adult mouse pituitary with FOXO1 plus LHB or TSHB primary antibodies. Proteins were visualized with secondary antibodies coupled to FITC or Texas Red. Representative images were obtained using a Nikon Eclipse TE 2000-U inverted fluorescence microscope at 40× magnification. Yellow arrows indicate colocalization, whereas white arrows show lack of colocalization. C and D, β-gal staining was performed on paraffin-embedded sections of adult mouse pituitary from Lhb-Cre x Rosa26 mice. IHC was then performed with a FOXO1 primary antibody (C). A black arrow indicates colocalization. The negative control (Neg) lacked primary antibody (D). Representative images were obtained using a Nikon Eclipse E800 microscope at 40× magnification.