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. 2012 Aug 3;287(40):33424–33435. doi: 10.1074/jbc.M112.362103

FIGURE 7.

FIGURE 7.

FOXO1 does not bind to the proximal Lhb promoter. TnT FLAG-FOXO1-CA was incubated with a consensus FBE, −150/−115, −122/−87, −95/−60, or −65/30 Lhb probes and tested for complex formation in EMSA. A, FOXO1-CA-DNA complex on the FBE is shown in lane 1, whereas anti-FLAG supershift is shown in lane 3, and IgG control is shown in lane 2. The FOXO1-CA-DNA complex and antibody supershift (ss) are indicated on the left of the gel. B, self-competition with excess cold FBE is shown in lane 4, and lack of competition with mutant FBE, −150/−115, −122/−87, −95/−60, or −65/−30 Lhb oligos is shown in lanes 5–9.