FIGURE 9.

FOXO1 suppresses basal and GnRH induction of the human LHB promoter. A and B, the −1068/+9 human LHB-luc reporter was transfected into LβT2 cells along with pcDNA3 empty vector (EV) or FOXO1 as indicated. After overnight starvation in serum-free media, cells were treated for 6 h with 0.1% BSA vehicle (Veh) or 10 nm GnRH. The results represent the mean ± S.E. of at least three experiments performed in triplicate and are presented as basal transcription relative to empty vector (A) or -fold GnRH induction relative to the vehicle control (B). * indicates that LHB-luc transcription is significantly repressed by FOXO1 compared with empty vector using Student's t test.