Skip to main content
. 2012 Aug 10;287(40):33436–33446. doi: 10.1074/jbc.M112.384578

FIGURE 1.

FIGURE 1.

p53 regulates TIGAR expression in human glioma. A, an in silico analysis was performed with the Oncomine database. TIGAR expression in normal brain samples was compared with glioblastoma patient samples (GBM); -fold change, 1.407, p value: 1.3 × 10−5. B, TIGAR expression was assessed by qRT-PCR in LNT-229p53V135A cells carrying the temperature-sensitive dominant-negative mutant p53V135A and hygro control cells (mean ± S.D., *, p < 0.05). rel. expression, relative expression. C, LNT-229 cells were treated with increasing concentrations of adriamycin for 20 h. p53 transactivation activity was determined by luciferase assay (shown is mean ± S.D. of triplicates, one experiment out of two independent experiments with similar results is shown, **, p < 0.01, unpaired Student's t test compared with untreated cells). Expression of endogenous human p53 was confirmed by Western blot. GAPDH expression was employed as loading control. D and E, expression of TIGAR (D) and TKTL1 (E) was determined by qRT-PCR (qPCR) in LNT-229 cells exposed to 345 ng/ml adriamycin for 20 h (shown is mean ± S.D. of triplicates, one experiment out of two independent experiments with similar results is shown, **, p < 0.01, unpaired Student's t test).