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. 2012 Aug 10;287(40):33436–33446. doi: 10.1074/jbc.M112.384578

FIGURE 2.

FIGURE 2.

TIGAR protects glioma cells from hypoxia-induced cell death. A–C, expression of TIGAR was assessed by qRT-PCR (qPCR) in LNT-229 transiently transfected with 100 nm scrambled siRNA (scr si) or TIGARsiRNA (TIGARsi) (A), T98G stably transfected with TIGAR-pcdna3.1 or pcdna3.1 plasmid (neo) (B), and LNT-229p53V135A stably transfected with TIGAR-pcdna3.1 or pcdna3.1 plasmid (neo) and hygro control cells transfected with pcdna3.1 (hygro/neo) (C). rel. expression, relative expression. D–F, these cells were exposed to serum-free medium containing 2 mm glucose and 0.1% O2 for 20 h, and cell death was analyzed by PI-FACS. T98G- and LNT-229-TIGAR-TM-pcdna3.1 cells were used as respective control (shown is mean ± S.D. of triplicates, one experiment out of three or more independent experiments with similar results is shown, *, p < 0.05, **, p < 0.01, unpaired Student's t test). n. s., not significant. G–I, cells were monitored by microscopy (×10) under these conditions.