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. 2012 Aug 10;287(40):33436–33446. doi: 10.1074/jbc.M112.384578

FIGURE 3.

FIGURE 3.

TIGAR protects glioma cells from ROS-induced cell death. A–C, LNT-229 transiently transfected with 100 nm scrambled siRNA (scr si) or TIGARsiRNA (TIGARsi) (A), T98G stably transfected with TIGAR-pcdna3.1 or pcdna3.1 plasmid (neo) (B), and LNT-229p53V135A cells stably transfected with TIGAR-pcdna3.1 or pcdna3.1 plasmid (neo) (C) were exposed to 1 mm H2O2 for 4 h (LNT-229) or 24 h (T98G), and cell death was assessed by PI-FACS (shown is mean ± S.D. of triplicates, one experiment out of three or more independent experiments with similar results is shown, **, p < 0.01, unpaired Student's t test). D–F, similarly, ROS were measured in the same cells by H2DCFDA-FACS and T98G- and LNT-229-TIGAR-TM-pcdna3.1 cells were used as respective control (shown is mean ± S.D. of triplicates, one experiment out of three or more independent experiments with similar results is shown, *, p < 0.05, **, p < 0.01, unpaired Student's t test). n. s., not significant.