TABLE 3.
LC50 (μm) for cyclotide induction of vesicle leakage
The peptide concentration needed to achieve 50% leakage (LC50) and the standard error were determined by fitting a non-linear regression equation (dose-response binding with variable slope), in which the maximum response was constrained to 100% leakage; see data and fitting in Fig. 7.
| Peptide | POPC/POPE (90:10) | POPC/POPE/POPG (70:10:20) | POPC/POPE/Chol/SM (17:10:33:40 ) |
|---|---|---|---|
| kB1 | 1.06 ± 0.04 | 5.77 ± 0.70 | 0.65 ± 0.04 |
| kB2 | 1.22 ± 0.12 | 2.94 ± 0.29 | 0.24 ± 0.01 |
| kB6 | 3.02 ± 0.83 | 22.45 ± 1.30 | 0.83 ± 0.17 |
| kB7 | 2.79 ± 0.01 | 6.86 ± 0.29 | 2.61 ± 0.08 |
| kB5 | 2.07 ± 0.16 | 2.47 ± 0.12 | 0.46 ± 0.01 |
| kB8 | 0.90 ± 0.08 | 0.89 ± 0.05 | 0.77 ± 0.04 |
| cyO2 | 0.24 ± 0.01 | 0.28 ± 0.01 | 0.21 ± 0.01 |
| tcA | 7.70 ± 0.38 | 18.50 ± 2.81 | 4.98 ± 0.21 |