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. 2012 Jul 18;287(40):33656–33663. doi: 10.1074/jbc.M112.375154

FIGURE 3.

FIGURE 3.

ESAT-6 induces Th2 cell differentiation via IL-1β production. A, IL-4 production in DCs co-cultured with naïve OT-II CD4+ T cells after infection with H37Rv or BCG in the presence or absence of anti-IL-1β. B, IL-4 production in DCs co-cultured with naïve OT-II CD4+ T cells after infection with H37Rv or mutant strains (H37RvΔRD1, H37RvΔESAT-6, and Comp ESAT-6) in the presence or absence of anti-IL-1β. IL-4 levels were higher in cultures with H37Rv or Comp ESAT-6 compared with cultures with BCG or other mutant strains (p < 0.003). C, Western blot analysis of STAT6, pSTAT6, STAT4, and pSTAT4 proteins from DCs infected with H37Rv or H37RvΔESAT-6 and co-cultured with OT-II CD4+ T cells, to show modulated Th1 and Th2 responses in the presence or absence of anti-IL-1β. The results shown are representative of at least four independent experiments. Error bars, ±S.D.