FIGURE 5.

IL-1β plays an important role in Th2 cell differentiation after infection with H37Rv without affecting Th1 cell differentiation. CD4⁺ T cells were isolated from congenic wild-type Thy1.2 or IL-1βR^−/− Thy1.2 mice and adoptively transferred into irradiated Thy1.1 congenic animals followed by infection with H37Rv. Thirty days after infection, spleen cells from these animals were isolated and challenged with H37Rv complete soluble antigen ex vivo. T cells were then stained for the intracellular cytokines IL-4 and IFN-γ. The results shown are representative of at least three independent experiments with three mice within each group.