FIGURE 4.

Characterization of several recombinant α-synuclein proteins as standards in the TR-FRET assay. A, six α-synuclein proteins from independent sources were plated in triplicate at the indicated concentrations on 384-well microtiter plates. TR-FRET analysis was with SynBa2-Tb/SynBa3-d2. Protein concentrations provided by the supplier were used to normalize protein input. B, Coomassie staining and Western blotting (WB) were used to evaluate the relative abundance of α-synuclein standards. C, shown is a table of identification and suppliers of recombinant standards.