FIGURE 1.

Solution structure of the CERT PH domain, determined by NMR. A, primary structure of CERT; B, backbone superposition of the 20 lowest energy structures; and C, ribbon diagram of the representative structure with the lowest energy. These molecular diagrams were generated with the program MOLMOL (26). D, electrostatic potential surface of the CERT PH domain. Red and blue indicate negative and positive electrostatic surfaces, respectively. The electrostatic potential was calculated using the program MOLMOL (26). The “front view” shows the same side as in B and C. E, ribbon model representation of the CERT PH domain, with magnification of the basic groove. The side chains of the basic and aromatic residues in the basic groove are displayed as blue and green sticks, respectively.