Anti-HIV activity assays for OAAHs. Single-cycle HIV-1 infectivity assays were performed using a luciferase reporter cell line to quantify the antiviral activity of the lectins. Experimental data points and the lines for CV-N (square), OAA (circle), PFA (diamond), MBHA (triangle), and OPA (inverted triangle) are colored in black, blue, green, red, and orange, respectively. Each data point was obtained from six duplicate measurements. The dashed black lines were used for extracting the IC50 concentration.