FIGURE 4.

Effect of NES1 mutation on PCNA subcellular localization. A, analysis of PCNA localization by indirect immunofluorescence using mouse monoclonal anti-HA antibody in HeLa cells transfected with pcDNA3-HA-PCNA constructs containing different mutations or deletions within the PCNA NES1. The cells were visualized using epifluorescence microscopy (original magnification ×400, scale bar = 20 μm). B, the percentages of cells showing strong nuclear PCNA localization (white bars, N>C) and pancellular localization (gray bars, N=C) are plotted in histograms. Counting was performed on a minimum of 100 transfected cells per experiment in three independent experiments. The data are the means ± S.E. of the three independent experiments, ***, p <0.001 (ANOVA test).