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. 2012 Aug 2;287(40):33826–33835. doi: 10.1074/jbc.M112.380113

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Analysis of IL-7 and anti-CD3 signaling in lentiviral vector-modified IL-7Rα^hi Molt3. A, surface expression of IL-7Rα on Molt3 (no infection) and lentiviral vector-modified IL-7Rα^hi Molt3 (+LV IL-7Rα). B, STAT5 activation in response to 100 ng/ml IL-7 by Phosflow antibody staining and flow cytometry analysis. C, surface expression of activation markers CD25 and CD69 in Molt3, IL-7Rα^hi Molt3, and Jurkat cells after overnight incubation with anti-CD3/CD28 beads. D, intracellular free calcium measurement in Indo-1/AM-loaded Molt3, IL-7Rα^hi Molt3, and Jurkat cells in response to anti-CD3 antibody (clone HIT3a) and ionomycin.