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. 2012 Aug 2;287(40):33826–33835. doi: 10.1074/jbc.M112.380113

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Live imaging and functional analysis of an IL-7Rα-red fluorescent protein fusion. A, functional IL-7Rα-mApple lentiviral vector construct with a helix-turn-helix hinge domain. CMV-TAR, cytomegalovirus-TAT transactivation response element; cPPT, central polypurine tract; hu, human; SIN-LTR/bGHpA, self-inactivating long terminal repeat/bovine growth hormone polyadenylation signal. B, fluorescence images of HeLa and Molt3 cells infected with IL-7Rα-hinge-mApple. C, STAT5 activation in response to IL-7 in Molt3 cells infected with lentiviral IL-7Rα-hinge-mApple.