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. 2012 Aug 1;287(40):33853–33864. doi: 10.1074/jbc.M112.395830

FIGURE 2.

FIGURE 2.

Nerve injury reduces the monomer and dimer KCC2 protein levels, but not KCC2 mRNA levels, in the dorsal spinal cord. a, Western blots (left) and quantification (right) of KCC2 (∼140 kDa) in the dorsal spinal cord ipsilateral (Ipsi) and contralateral (Cont) to SNL (n = 8) are shown. b, Western blots (left) and quantification (right) of protein levels of the monomer and dimer KCC2 in the dorsal spinal cord ipsilateral (Ipsi) and contralateral (Cont) to SNL (n = 6) are shown. The tissue sample was subjected to 6% polyacrylamide gel, and the KCC2 protein level was normalized to that of GAPDH in the same sample. c, nerve injury does not change the KCC2 mRNA level in the spinal cord. The histogram shows quantification of KCC2 mRNA levels in the dorsal spinal cord ipsilateral (Ipsi) and contralateral (Cont) to SNL 4 weeks after SNL (p = 0.39, Mann-Whitney test). The KCC2 mRNA level was normalized by that of S18 rRNA (n = 6). d, shown are Western blots (inset) and quantification of KCC2 levels in the dorsal spinal cord from control and sham-operated rats (n = 6 in each group). * p < 0.05, ** p < 0.01, Mann-Whitney test. Error bars represent S.E.