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. 2012 Sep 8;8(4):711–718. doi: 10.5114/aoms.2012.30295

Figure 1.

Figure 1

Purification of TAT-CAIII under native conditions. M – Pre-stained protein molecular weight marker. A – Protein expression of pET28a-TAT-CAIII transformed bacteria (1 – total proteins before being induced by IPTG; 2 – total proteins after being induced by IPTG; 3 – proteins in the supernatants after being induced by IPTG). B – Purification of the TAT-CAIII fusion protein (1 – cell supernatants; 2 – flow-through; 3 – Ni-buffer-20 wash; 4 – Ni-buffer- 50 wash; 5 – Ni-buffer-100 wash; 6 – Ni-buffer-250 elution). C – (1 – Purified TAT-CAIII)