Figure 2.

Identification of the purified products and determination of the TAT-CAIII transmembrane ability. A – Ponceau staining, western blot identification and phosphatase activity staining of the purified products (a – Ponceau staining; b – Reacting with the anti-CAIII polyclonal antibody; c – Reacting with the anti-His-tag monoclonal antibody; d – Phosphatase activity staining). M – Pre-stained protein molecular weight marker; 1 – Purified TAT-CAIII; 2 – Purified CAIII. B – Identification of the TAT-CAIII transmembrane ability (200×)