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. Author manuscript; available in PMC: 2012 Sep 28.
Published in final edited form as: Nature. 2011 Apr 28;472(7344):476–480. doi: 10.1038/nature09973

Figure 2. TRAF6 is recruited to mitochondria upon TLR1/2/4, but not TLR3/9, signaling to engage ECSIT on the mitochondrial surface.

Figure 2

a-e, RAW cells were stimulated with TLR agonists for the indicated times, cells were fractionated, and extracts were blotted. Purified mitochondrial lysates were immunoprecipitated with ECSIT antibody overnight (d). Heavy chain IgG is indicated by an asterisk (d). Equal amounts of extracts were treated with the indicated amount of proteinase K on ice with or without 0.2% saponin to gently permeabilize mitochondrial membranes (e).