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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cancer Res. 2012 Feb 20;72(7):1890–1900. doi: 10.1158/0008-5472.CAN-11-3472

Figure 2. Jab1 is overexpressed and regulates p27 levels in NPC cells.

Figure 2

(A) Whole-cell lysates were prepared from the cells as indicated. β-actin was used as a control for protein loading and integrity. The relative Jab1 and p27 intensity from 5 samples is shown. (B) Proteolysis of p27 in NPC cells is sensitive to proteasome inhibitors. (C) Inducible expression of Ad-Myc-Jab1 (Tet-off) downregulates endogenous p27 levels in NPC cell lines. Lysates were prepared from Ad-Myc-Jab1-infected cells in the absence (−) or presence (+) of doxycycline (Dox). (D) NPC cells were transduced with the Ad-Myc-Jab1 virus, and treated as indicated with proteasome inhibitor. The protein levels were quantified using ImageJ software.