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. Author manuscript; available in PMC: 2012 Sep 28.
Published in final edited form as: Cell Metab. 2007 Apr;5(4):265–277. doi: 10.1016/j.cmet.2007.02.009

Figure 1. Deletion of TOR1 Extends Chronological Life Span and Increases Respiration in Two Different Yeast Strains.

Figure 1

(A) Chronological life-span curves (i.e., % viability versus days in stationary phase) of wild-type (closed circles) and tor1 null cells (open circles) over two weeks, as assessed by staining with trypan blue. The curves shown were generated by compiling survival measurements from 11 different samples performed in 5 independent experiments. For clarity, we have marked the approximate median survival point of each curve with a dotted line. In this and all other figures, error bars represent standard deviation unless otherwise noted.

(B) Serial 10-fold dilutions of stationary-phase cultures plated onto rich medium (YPD) at day 1 (upper panel) and day 21 (lower panel).

(C) Chronological life-span measurements of wild-type and tor1 null derivatives of the short-lived strain BY4742 plotted as described in (A). *p < 0.05; **p < 0.005; ***p < 0.0005.

(D) Chronological life-span curves of wild-type and tor1 null cells treated with 50 nM rapamycin or drug vehicle (ethanol). Survival was monitored by trypan blue staining.

(E) Mitochondrial oxygen consumption of wild-type and tor1 null derivatives of strains DBY2006 (left) and BY4742 (right) at day 1 postinoculation in SD medium.