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. Author manuscript; available in PMC: 2012 Sep 28.
Published in final edited form as: Nat Struct Mol Biol. 2010 Nov 21;17(12):1406–1413. doi: 10.1038/nsmb.1941

Figure 1.

Figure 1

Estrogen induction increases histone acetylation at the BRCA1 promoter. Top panel: a schematic illustration of the bidirectional promoter of the BRCA1/NBR2 gene locus showing positions of the ChIP amplicons. (a) BRCA1 nascent and mature RNA expression in control or MCF-7 cell treated 24 h with 10 nM estradiol (E2). Error bars indicate the s.e.m. of N=3 biological replicates. (b–f) ChIP profiles of resting and E2 stimulated MCF-7 cells using antibodies against Pol II (b), p300 (c), CREB (d), acetylated histone H4 (e), and acetylated histone H3 (f) at the BRCA1 promoter. Error bars represent the s.e.m. for N=3 (Pol II), N=2 (p300), N=2 (CREB), N=3 (AcH4) and N=3 (AcH3) biological replicates.