Amount	Component	Final Concentration
190 ml	DMEM (low glucose)	N/A
10 ml	fatty acid free BSA (20mg/ml in DMEM)	50 μg/ml
2.8 ml	L-Glutamine	1.4 mM
4 ml	insulin/transferrin/selenium(100x)	10 μg/ml insulin 5.5 μg/ml transferrin 38.7 nM selenium
0.4 ml	NGF (125 μg/ml)	100 ng/ml
200 ml	Ham F-12	N/A