| Component | Volume (μl) | Final |
| Nuclease free water* | X | |
| 10X Reaction Buffer | 2 | 1X |
| ATP (100 mM) | 2 | 10mM |
| CTP (100 mM) | 2 | 10mM |
| UTP (100 mM) | 2 | 10mM |
| GTP (20 mM) | 2 | 2mM |
| 3´-0-Me-m7G(5')ppp(5')G cap analog (ARCA)(40mM) | 4 | 8mM |
| Template DNA* | X | 1 μg |
| T7 RNA Polymerase Mix | 2 | |
| Total | 20 |
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