FIG. 1.

The embryonic chick femur organotypic culture set up. (A) 0.44 μm filter membrane inserts positioned in six-well plates. (B) Embryonic chick femurs were isolated and placed on the membrane and (C) culture media was then added to the six-well plates to create an air/liquid interface. Cell viability and histological analysis of organotypic cultured femurs. Cell viability (cell tracker green (CMFDA)/ethidium homodimer-1) of embryonic chick femurs (E13) organotypic cultured in (D) osteogenic conditions for 10 days and examined by fluorescent microscopy showing viable cells (green) and negligible associated necrosis (red). (E) A representative tissue section from (E13) embryonic chick femur cultured in osteogenic conditions displaying viable cells throughout the organotypic cultured femur (scale bar=100 μm). Color images available online at www.liebertonline.com/tec