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. 2012 May 14;18(10):747–760. doi: 10.1089/ten.tec.2012.0033

FIG. 6.

FIG. 6.

μCT and histological analysis of organotypic cultured embryonic femurs (E12) in basal, chondrogenic, and osteogenic conditions. (A) Femur lengths; ***p<0.001 increase in femur length compared with noncultured femurs. (B) μCT morphometric indices of the structure of E12 embryonic chick femurs organotypic cultured in basal, chondrogenic, and osteogenic media for 10 days. Values are mean±SD (n=4 femurs per group). μCT morphometric indices of the structure of the organotypic cultured embryonic chick femurs E12 (NC=noncultured, B=basal, C=chondrogenic, O=osteogenic). Values are mean±SD (n=4 femurs per group). *p<0.05 ***p<0.001 increase or decrease in μCT bone morphometric indices of basal, chondrogenic, and osteogenic cultured femurs compared to noncultured (NC) femurs. (C) Histological analysis of the E12 embryonic femurs cultured in the three conditions for Alcian blue/Sirius red, von Kossa, expression of collagen type I and II and STRO-1+ (scale bar=100 μm). Color images available online at www.liebertonline.com/tec