Skip to main content
. 2012 Sep 27;8(9):e1002929. doi: 10.1371/journal.ppat.1002929

Figure 4. Effects of silencing the expression of Nup98 or Rae1 on complexes containing Rae1.

Figure 4

HeLa cells were transfected with Nup98 siRNA (A), Rae1 siRNA (C), or non-targeting siRNA (D). 48 hours post-transfection, lysates were chromatographed on a Superdex 200 column. Fractions were analyzed by immunoblots probed for the indicated proteins. (B) and (E): Column fractions from (A) and (D), respectively, were incubated with GST-M protein (M) or GST (G) on glutathione beads for 1 hour, and bound fractions were analyzed by immunoblots probed for Rae1 and Nup98.