Figure 9. Effect of silencing Rae1 expression on the inhibition of host gene expression by M protein expressed in the absence of other VSV components.

HeLa cells were either not transfected or transfected with Rae1 siRNA or non-targeting (NT) siRNA. After 48 hours, cells were re-seeded, then cotransfected with plasmid DNA encoding luciferase under the control of the SV40 promoter together with the indicated amounts of M mRNA. The amount of total RNA was kept constant by addition of yeast tRNA. At 24 hours post-transfection, cells were lysed and luciferase reporter activity was measured. The graph represents activity expressed as a percentage of average luciferase activity in the absence of M mRNA. Data shown are means ± standard deviation from three independent experiments.