Table 1. Effect of silencing Rae1 or Nup98 expression on RNA synthesis in VSV-infected cells.
| A. Rae1 | 3H cpm (×10−3) ± s.d. | % of mock | ||||
| siRNA | Infection | −Act Da | +Act Da | Difference | Host | Viral |
| None | Mock | 54.1±19.4 | 0 | 54.1 | 100 | - |
| VSV | 9.4±0.2 | 2.4±0.8 | 7.0 | 12.9 | 4.4 | |
| NTa | Mock | 96.2±48.1 | 0 | 96.2 | 100 | - |
| VSV | 4.7±1.7 | 1.5±0.2 | 3.2 | 3.3 | 1.6 | |
| Rae1 | Mock | 50.8±8.5 | 0 | 50.8 | 100 | - |
| VSV | 28.9±14.3 | 2.6±0.7 | 26.3 | 51.8 | 5.1 | |
HeLa cells transfected with the indicated siRNA were either mock-infected or infected with VSV in the presence or absence of actinomycin D. At 6 h postinfection, cells were labeled with 3H uridine for 30 min; RNA was acid-precipitated, and radioactivity was determined by scintillation counting. Data shown are mean ± s.d. for triplicate cultures from a representative experiment.
a
Abbreviations: ActD - actinomycin D; NT – non-targeting siRNA.