Figure 2. Functional requirement testing and mapping.

A. Required regions were defined as regions with a statistical underrepresentation of insertion counts compared to the rest of the genome. To test this, we applied a non-parametric test for regions of increasing size, as described in B. Every 250, 400, 500, and 600 bp region (large enough for statistical power) was tested for insertion count underrepresentation, generating a comprehensive map of required regions in the Mtb genome. Tracks on the circularized genome, from inner-most to outer-most: 1. Histogram of insertion counts, 2. Annotated genes, forward direction, 3. Annotated genes, reverse direction, 4. All required regions. 5. Required intergenic regions.