Table 1.

Summary of histopathologic analysis of leukemic infiltration in brain

Treatment	CNS symptoms	Death	Striatum	Thalamus	Brainstem/pons	Cerebellum
None*	No	Killed	0	0	0	0
None*	No	Radiation	0	0	0	0
None	No	Leukemia	0	0	0	0
Dasatinib	No	Leukemia	1	1	1	2
Dasatinib	No	Leukemia	1	1	1	1
Dasatinib	Yes	Leukemia	3	2	4	2
Dasatinib + plerixafor	Yes	Killed because of neurosymptoms	2	2	2	4
Dasatinib + plerixafor	Yes	Killed because of neurosymptoms	1	3	4	1
Dasatinib + plerixafor	Yes	Killed because of neurosymptoms	3	3	4	4

The presence of GFP-positive infiltrates in various brain structures, as established by anti-GFP immunohistochemistry with a semiquantitative scheme of assessment: 1 indicates perivascular leukemic infiltrates; 2, nodular infiltrates < 0.5 mm; 3, nodular infiltrates 0.5-1 mm; and 4, nodular infiltrates > 1 mm. The presence of CNS symptoms was most closely associated with involvement of the brainstem and pons by nodular leukemic infiltrates, although the number of animals undergoing necropsy examination was too small to establish a statistically significant association between treatment and specific pathologic findings.

^*Nonleukemic mice that were killed to use as controls.