RT-PCR study showing expression of differentiation and stem-cell markers (CK3, CK12, CK13, ABCG2, and p63).
Notes: The presence of CK3, CK12, and CK13 indicates the nonkeratinized corneal epithelial cell property, and ABCG2 and p63 indicate the stem-cell property of the limbal cultivated cells. The findings are identical to that of the immunohistochemistry study.
Abbreviations:
ABCG2, ATP-binding cassette subfamily G member 2; Bp, base pair; CK3, cytokeratin 3; CK12, cytokeratin 12; CK13, cytokeratin 13; p63, transformation-related protein 63; RT-PCR, reverse-transcription polymerase chain reaction.