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. 2012 Apr 27;12:14. doi: 10.1186/1471-213X-12-14

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Copyright ©2012 Sutherby et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pre-treatment of larvae with histamine (HA) leads to an increase of metamorphic competence based on a settlement induction assay with KCl (A) or settlement plates (SP) (B). Treatment of competent larvae with histamine (HA) receptor 1 antagonist (125 μM chlorpheniramine) and receptor 3 antagonist (125 μM thioperamide) leads to a reduction of arm length measured by the length of the exposed skeletal element from the extent of the arm flesh. HA receptor 2 antagonist (200 μM cimetidine) and HA (1 μM) did not significantly affect relative arm length (A). HA alone did not affect relative arm length in either experiment. Panel B illustrates the measurement of the arm flesh length subtracted from the skeletal element length, giving total exposed skeletal rod length. Note that larval arm length was measured within 24 hours of exposure to treatment. Panel C shows representative larvae that were measured in these experiments. Scale bar: 150 μm.