Table 1.
Sequence-specific Primers | Primer sequence | Product size (bp) |
---|---|---|
G/C allele at position−137 |
|
|
Common reverse primer |
5’–AGGAGGGCAAAATGCACTGG–3’ |
|
forward primers 1 |
5’–CCCCAACTTTTACGGAAGAAAAG–3’ |
261 |
forward primers 2 |
5’–CCCCAACTTTTACGGAAGAAAAC–3’ |
261 |
Control forward primer |
5’–CCAATAGGACTGATTATTCCGCA–3’ |
446 |
C/A allele at position−607 |
|
|
Common reverse primer |
5’–TAACCTCATTCAGGACTTCC–3’ |
|
forward primers 1 |
5’–GTTGCAGAAAGTGTAAAAATTATTAC–3’ |
196 |
forward primers 2 |
5’–GTTGCAGAAAGTGTAAAAATTATTAA–3’ |
196 |
Control forward primer | 5’–CTTTGCTATCATTCCAGGAA–3’ | 301 |