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. 2012 Apr 24;10:31. doi: 10.1186/1477-7827-10-31

Table 1.

Sequence-specific primers for G/C and C/A alleles and their PCR product sizes for positions −137 and −607 in the promoter of theIL-18gene

Sequence-specific Primers Primer sequence Product size (bp)
G/C allele at position137
 
Common reverse primer
5’–AGGAGGGCAAAATGCACTGG–3’
 
forward primers 1
5’–CCCCAACTTTTACGGAAGAAAAG–3’
261
forward primers 2
5’–CCCCAACTTTTACGGAAGAAAAC–3’
261
Control forward primer
5’–CCAATAGGACTGATTATTCCGCA–3’
446
C/A allele at position607
 
Common reverse primer
5’–TAACCTCATTCAGGACTTCC–3’
 
forward primers 1
5’–GTTGCAGAAAGTGTAAAAATTATTAC–3’
196
forward primers 2
5’–GTTGCAGAAAGTGTAAAAATTATTAA–3’
196
Control forward primer 5’–CTTTGCTATCATTCCAGGAA–3’ 301