Table 3.
Relative expression of genes assayed by RT-qPCR
|
Genes |
VO/FO |
Lean/Fat |
||||||
|---|---|---|---|---|---|---|---|---|
| |
Lean |
Fat |
FO |
VO |
||||
| Ratio | p value | Ratio | p value | Ratio | p value | Ratio | p value | |
|
Microarray validation | ||||||||
| Δ5fad (D) |
1.72 |
ns |
4.34 |
0.005 |
2.49 |
ns |
−1.01 |
ns |
| NADH1 (D) |
−1.03 |
ns |
−1.28 |
0.05 |
1.00 |
ns |
1.24 |
ns |
| UCP2 (D) |
−1.10 |
ns |
1.08 |
ns |
−1.10 |
ns |
−1.28 |
0.003 |
| MTHFD1 (D) |
1.20 |
ns |
−1.09 |
ns |
−1.08 |
ns |
1.22 |
ns |
| PA2G4 (D) |
−1.27 |
ns |
−1.23 |
0.032 |
1.11 |
ns |
1.1 |
ns |
| RP60S (D) |
−1.02 |
ns |
−1.69 |
0.023 |
1.13 |
ns |
1.9 |
0.006 |
| MYO (D) |
1.77 |
0.034 |
1.22 |
ns |
−1.35 |
ns |
1.08 |
ns |
| PCNA (D) |
−1.14 |
ns |
−1.27 |
ns |
1.08 |
ns |
1.20 |
ns |
| CYP1A (D+G) |
1.71 |
0.004 |
1.81 |
0.023 |
−2.22 |
0.003 |
−2.38 |
0.001 |
| ABCA1 (G) |
1.24 |
ns |
1.17 |
ns |
1.21 |
ns |
1.28 |
0.015 |
| PSMB8 (G) |
−100.00 |
ns |
1.87 |
0.033 |
−16.67 |
0.026 |
< −1000 |
0.000 |
| TAGLN (G) |
1.23 |
ns |
−1.02 |
ns |
−1.03 |
ns |
1.22 |
ns |
| COL1A2 (G) |
1.31 |
ns |
−1.06 |
ns |
1.47 |
ns |
2.06 |
0.001 |
|
LC-PUFA biosynthesis and regulation | ||||||||
| Δ6fad_a |
1.11 |
ns |
2.96 |
0.008 |
1.76 |
ns |
−1.52 |
ns |
| elovl5a |
1.08 |
ns |
1.2 |
ns |
1.03 |
ns |
−1.08 |
ns |
| elovl5b |
−1.19 |
ns |
1.92 |
0.031 |
1.54 |
ns |
−1.47 |
ns |
| elovl2 |
1.2 |
ns |
14.74 |
0.003 |
4.89 |
ns |
−2.50 |
ns |
|
Fatty acid synthesis | ||||||||
| FAS |
1.49 |
0.010 |
1.20 |
ns |
1.05 |
ns |
1.31 |
ns |
|
Fatty acid oxidation | ||||||||
| ACO |
1.18 |
ns |
1.28 |
ns |
1.19 |
ns |
1.09 |
ns |
| CPT1 |
1.23 |
ns |
−1.09 |
ns |
−1.16 |
ns |
1.15 |
ns |
|
Regulation of lipid metabolism | ||||||||
| PPARα |
1.14 |
ns |
1.38 |
ns |
1.26 |
ns |
1.04 |
ns |
| PPARβ |
1.03 |
ns |
1.03 |
ns |
−1.02 |
ns |
−1.02 |
ns |
| PPARγ |
1.52 |
ns |
1.60 |
0.036 |
1.21 |
ns |
1.15 |
ns |
|
Xenobiotic and oxidative stress | ||||||||
| GST |
−1.30 |
ns |
1.07 |
ns |
1.21 |
ns |
−1.14 |
ns |
| MT-A |
−1.82 |
0.051* |
−1.05 |
ns |
−1.25 |
ns |
−2.13 |
0.007 |
| CAT |
1.57 |
0.036 |
1.26 |
ns |
−1.52 |
ns |
−1.22 |
ns |
| SOD |
−1.20 |
ns |
−1.04 |
ns |
1.21 |
ns |
1.04 |
ns |
|
Apoptosis | ||||||||
| CASP3B |
1.22 |
0.045 |
1.22 |
ns |
−1.04 |
ns |
−1.04 |
ns |
| CASP6A/B | 1.30 | ns | 1.37 | ns | −1.14 | ns | −1.20 | ns |
fad: Fatty acyl desaturase (Δ5 and Δ6 activities); NADH1: NADH dehydrogenase subunit 1; UCP2: Uncoupling protein 2; elovl: MTHFD1: Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like; PA2G4: Proliferation-associated 2G4b; RP60S: 60S acidic ribosomal protein; MYO: Myosin heavy chain; PCNA: Proliferating cell nuclear antigen; CYP1A: Cytochrome P4501A; ABCA1: ATP-binding cassette sub-family A member 1; PSMB8: Proteasome subunit beta type-8 precursor; TAGLN: Transgelin 2; COL1A2: Collagen type I alpha 2; elovl2: Elongase 2; FAS: Fatty acid synthase; ACO: Acyl-CoA oxidase; CPT1: Carnitine palmitoyltransferase I; PPARs; Peroxisome proliferator-activated receptors; GST: glutathione S-transferase; MT-A: Metallothionein A; CAT: catalase; SOD: superoxide dismutase; CASP; caspase.
*Only marginally non-significant.
Genes correspond to features significantly affected by either diet (D) or genotype (G) in the microarray analysis or involved in biological processes being analysed in further detail. Values are normalized (by cofilin-2 and elf-1 α) gene expression ratios between fish fed VO in relation to FO for each family group or of Lean fish in relation to the Fat group when fed either one of the diets. Values in bold are significantly different, at p<0.05 (REST 2008); ns - non-significant.