Table 5.
Proteins differentially regulated by diet
| Spot No | Protein ID | Accession No. (NCBInr) | Theoretical Mw (KDa)/pI | Protein Scorea | No. Matched peptides (MS/MS)b | Best score peptide | VO/FO Lean | VO/FO Fat | p-value |
|---|---|---|---|---|---|---|---|---|---|
| 1136 |
Epoxide hydrolase 2 (EPHX2) |
gb|ACI33129.1| |
54.2/5.52 |
84 |
1 |
GGLFVGLPDEIPR |
1.23 |
1.40 |
0.0190 |
| 1148 |
Hemopexin-like protein* (HPX) |
emb|CAA92147.1| |
50.4/5.61 |
169 |
1 |
VHLDAITSDDAGNIYAFR |
1.32 |
1.56 |
0.0002 |
| 1151 |
Hemopexin-like protein* (HPX) |
ref|NP_001104617.1| |
51.0/6.18 |
161 |
1 |
VHLDAITSDDAGNIYAFR |
1.38 |
1.54 |
0.0001 |
| 2683 |
Peroxiredoxin 1 (PRDX1) |
gb|ACI67145.1| |
22.0/6.42 |
757 |
8 |
SISTDYGVLKEDEGIAYR |
1.14 |
1.09 |
0.0310 |
| 3168 |
2-peptidylprolyl isomerase A (PPIA) |
ref|NP_001135161.1| |
17.5/7.61 |
101 |
1 |
VYFDITIGDTPAGR |
−1.46 |
−1.18 |
0.0170 |
| 3243 |
Myosin light chain smooth muscle isoform (MYL) |
ref|NP_998803.1| |
16.9/4.47 |
100 |
2 |
EAFLLFDR |
−1.23 |
−1.11 |
0.0490 |
| 3331 |
similar to H2A histone family, member J (H2A) |
ref|XP_001521566.1| |
13.3/10.84 |
74 |
1 |
AGLQFPVGR |
−1.58 |
−1.18 |
0.0250 |
| 3334 |
Histone H2A** (H2A) |
emb|CAA25528.1| |
13.7/10.88 |
37 |
1 |
AGLQFPVGR |
−1.48 |
−1.18 |
0.0210 |
| 3445 | Galectin 2 (LGALS2) | gb|ACN10131.1| | 14.8/5.93 | 314 | 2 | SGASSFSINIGHDSDNYALHFNPR | 1.17 | 1.15 | 0.0170 |
a The protein score probability limit (where P < 0.05) is 73.
b Peptides with confidence interval above 95% were considered.
* Equivalent to warm-temperature-acclimation-related-65 kDa-protein.
**Same protein identification obtained by MS/PMF (peptide mass fingerprinting): Score = 76; 7 peptides matched.
Only reliable identifications of Actinopterygii, obtained by mass spectrometry (MALDI-TOF-MS/MS) analysis and searches in MASCOT, NCBI and ExPASy (Mw/pI) databases are shown. Expression ratios between fish fed VO and FO diets are given for each family group, as well as p-value for diet (two-way ANOVA, DeCyder V7.0).