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. Author manuscript; available in PMC: 2012 Sep 28.
Published in final edited form as: Dev Dyn. 2011 Mar 29;240(6):1548–1557. doi: 10.1002/dvdy.22622

Fig. 2. A single morpholino targets a matched sequence in both tbx2 genes.

Fig. 2

Embryos were injected at the one cell stage with 100 pg of tbx2a-gfp (A-D) or tbx2b-gfp (E-F) RNA which leads to strong fluorescence throughout the embryo at 24 hpf (A, C, E). This signal is completely blocked by co-injection of MO2a (B) or MO2ab (D, F). Note that the previously validated MO2b is not used in this assay, because it is a splice-blocker. However, MO2a does not block expression of the tbx2b-gfp transcript (not shown). For each sample n>25.