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. 2012 Sep 28;7(9):e46225. doi: 10.1371/journal.pone.0046225

Figure 2. Cg-Ppm2 exhibits apolipoprotein N-acyltransferase activity. A.

Figure 2

Localization of AmyE in C. glutamicum Δppm2 or Δppm2 (Cg-ppm2) strains. Membrane (M) and secreted (S) proteins were analyzed by SDS-PAGE followed by immunoblotting using monoclonal anti-his antibodies. The band labeled with an asterisk corresponds to a shorter form of AmyE. B. MALDI PMFs of AmyE protein purified from ΔCg-ppm2 and ΔCg-ppm2 (Cg-ppm2) strains. The m/z 3200–4800 region of the mass spectra of AmyE tryptic peptides after DDM/CHCl3-CH3OH treatment is shown and significant monoisotopic [M+H]+1 peaks are annotated. Upper panel: m/z 3858.98 (bold) corresponds to the diacylated AmyE1–29 peptide while m/z 3451.65, 3547.59 and 4045.88 match to internal tryptic peptides, AmyE334–365, AmyE60–91 and AmyE55–91, respectively. Bottom panel: m/z 4097.21 (bold) corresponds to the triacylated AmyE1–29 peptide while m/z 3451.65, 3547.61 and 4045.88 match to AmyE334–365, AmyE60–91 and AmyE55–91 peptides.