Table 1. Summary of the N-terminal LppX1–29 peptides detected by MALDI-PMF after DDM/CHCl3-CH3OH treatment in different genetic backgrounds.
Strains | Triacylated LppX1–29 MH+ = 3778.46 | Triacylated and glycosylated LppX1–29 (triacylated LppX + n×162) | Diacylated LppX1–29 MH+ = 3540.46 | Diacylated and glycosylated LppX1–29 (diacylated LppX+ n×162) |
WT | 4103.09 (n = 2) | |||
4427.11 (n = 4) | ||||
Δppm1 | 3779.28 | |||
Δppm1(pCg-ppm1) | 4103.03 (n = 2) | |||
4427.05 (n = 4) | ||||
Δppm1(pMt-ppm1/D1+D2) | 4103.22 (n = 2) | |||
4427.18 (n = 4) | ||||
Δppm2 | 3540.89 | |||
Δppm2(pCg-ppm2) | 3779.23 | 3941.15 (n = 1) | ||
4103.22 (n = 2 ) | ||||
Δppm2(pMt-ppm1/D1+D2) | 3779.20 | 3941.22 (n = 1) | ||
4103.26 (n = 2) | ||||
4427.28 (n = 4) | ||||
Δppm2(pCg-ppm1) | 3864.99 (n = 2) | |||
4188.82 (n = 4) | ||||
Δppm2(pMt-ppm1/D1) | 3702.89 (n = 1) | |||
3864.87 (n = 2) |
Triacylated peptides are in bold. Glycosylated peptides are in italic. n represents the number of hexoses associated with the peptide.
Unmodified LppX 1–29 MH+ = 2964.46.