Table 1. Leaf biomass, chlorophyll content and PSI to PSII ratio of Col-0, Ws-4, and Ler-0 accessions.
Accession | Shoot weight | Leaf specific mass | Leaf chlorophyll contenta | PSI/PSIIb | ||
g plant−1(n = 11–18) | mg cm−2(n = 8–15) | µg cm−2(n = 8–15) | mg g−1(n = 8–15) | Chl a/b(n = 8–15) | (n = 3) | |
Col-0 | 5.72±0.69100% | 16.77±1.18100 | 49.84±5.34100% | 2.97±0.12100% | 4.00±0.26 | 1.06±0.09 |
Ws-4 | 7.38±0.68*130% | 13.32±0.47*79% | 41.19±4.79*83% | 3.09±0.17104% | 3.80±0.04 | 1.11±0.02 |
Ler-0 | 4.42±0.83*77% | 17.39±1.90103% | 53.68±3.37108% | 3.08±0.28103% | 3.86±0.29 | 1.12±0.08 |
Leaf chlorophyll content was measured spectrophotometrically after extraction in ethanol, and expressed on leaf area and on fresh weight basis.
PSI/PSII ratio was measured by EPR spectroscopy in isolated thylakoids.
The data were expressed as means ±SD (n = number of replicates). The parameters were also expressed relative to Col-0. *, Significantly different from Col-0 (Student's t-test P<0.05).
The parameters were measured on plants grown hydroponically for six weeks at an irradiance of 120 µmol photons m−2 s−1.