Table 3. Quantitative LC-MS of PSII core protein phosphorylation of Col-0, Ws-4 and Ler-0 accessions.
| Protein | Col-0 | Ws-4 | Ler-0 |
| P-CP43 | 100% | 100±7% | 100±14% |
| P-D1 | 100% | 50±12%* | 100±15% |
| P-D2 | 100% | 48±8%* | 98±8% |
| P-PsbH | 100% | 95±11% | 102±9% |
| PP-PsbH | 100% | 96±12% | 100±16% |
Data represent means ±SD of four independent preparations.
*
, Significantly different from Col-0 and Ler-0 (Student's t-test P<0.05).
The plants were grown hydroponically for six weeks at an irradiance of 120 µmol photons m−2 s−1 and treated for 3 h at an irradiance of 950 µmol photons m−2 s−1. Thylakoid membranes were isolated in the presence of NaF. The levels of phosphorylated PSII core proteins were analyzed by quantitative mass spectrometry and expressed relative to Col-0.