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. 2012 Jul 1;5(4):362–369. doi: 10.4161/cib.20215

Table 4. Oligonucleotides.

Oligonucleotide Describtion Sequence Restriction site
18C_seq_fwd
pUT18C sequencing
gttcgaagttctcgccggatg
 
18C_seq_rev
pUT18C sequencing
cagcgggtgttggcgggtgtc
 
18N_seq_fwd
pUT18 sequencing
caacgcaattaatgtgag
 
18N_seq_rev
pUT18 sequencing
acgcgcctcggtgcccac
 
glpF_BTH_fwd
glpF - > BTH
 
cattctagagatgacagcattttggggaga
XbaI
glpF_BTH_rev
glpF - > BTH
 
catggtacccgaatatatttagaatttgata
KpnI
pksL_BTH_fwd
pksL - > BTH
 
cattctagagatgaggtggaggtctaacgt
XbaI
pksL_BTH_rev
pksL - > BTH
 
catggtacccgtccgactaatgtataatcct
KpnI
yneK_BTH_fwd
yneK - > BTH
 
catgtcgactatgctggaaggatggttttt
SalI
yneK_BTH_rev
yneK - > BTH
 
catggtacccgtttgagaagggtctgataagg
KpnI
ymdA_BTH_fwd
ymdA - > BTH
 
cattctagagatgaccccaattatgatggt
XbaI
ymdA_BTH_rev
ymdA - > BTH
 
catggtacccgttttgcatactctacggctcgagtc
KpnI
ydhI_BTH_fwd
ydhI - > BTH
 
cattctagagatgatgatcatcatcccaaacaatg
XbaI
ydhI_BTH_rev
ydhI - > BTH
 
catggtacccgatcaattaccttcgaaaata
KpnI
ywpG_BTH_fwd
ywpG - > BTH
 
cattctagagatgaatcaatttcgtttaaa
XbaI
ywpG_BTH_rev
ywpG - > BTH
 
catggtacccggcctctgtttttatctttcgttttc
KpnI
ybfI_BTH_fwd
ybfI - > BTH
 
cattctagagatgcaaaacgaaacccgcac
XbaI
ybfI_BTH_rev
ybfI - > BTH
 
catggtacccgtctgtgaagctccttttcaa
KpnI
dltE_BTH_fwd
dltE - > BTH
 
cattctagagatgaagatgacaaataatac
XbaI
dltE_BTH_rev
dltE - > BTH
 
catggtacccgattctcctgcgtgttcatttgctcg
KpnI
hit_BTH_fwd
hit - > BTH
 
cattctagagatgcattgtgcagagaattg
XbaI
hit_BTH_rev
hit - > BTH
 
catggtacccgtgatgaggccaggcgttttgcgata
KpnI
yneK_for
yneK- gfp
 
catctcgagatgctggaaggatggtttttatg
XhoI
ynk_rev
yneK- gfp
 
cataagctttttgagaagggtctgataagg
HindIII
ymdA_for
ymdA- gfp
 
catgtcgacatgaccccaattatgatgg
SalI
ymdA_rev
ymdA- gfp
 
catgaattcttttgcatactctacggctc
EcoRI
ywpG_for
ywpG- gfp
 
catctcgagatgaatcaatttcgtttaaaag
XhoI
ywpG_rev
ywpG- gfp
 
cataagcttgcctctgtttttatc
HindIII
Ypbr-B-2-H-F
dynA- > BTH
 
cattctagagacagatcaaaacag
XbaI
Ypbr-B-2-H-R
dynA- > BTH
 
catggtaccatttttattgtattgtctg
KpnI
D1_B2H_rev
D1 - > BTH
 
catggtaccctatcaagccttttcaccct
KpnI
D2_B2H_fwd D2 - > BTH   cattctagagatgccgaaatcagaaatcaaaa XbaI