Figure 1. Involvement of LIMK1 in actin polarization in human CD4 T cells. (A) shRNA-mediated LIMK1 knockdown in CEM-SS T cells. Cells carrying stable LIMK1 knockdown or shNTC (a control shRNA against no human genes) were selected in puromycin and analyzed by western blot using antibodies against human phospho-cofilin, cofilin, LIMK1/2 or GAPDH (Bulk, bulk cell populations; clone 007, a derived LIMK1 knockdown cell clone). (B and C) LIMK1 knockdown decreases F-actin in clone 007. The decreases of F-actin in the knockdown cells were measured by FITC-phalloidin staining and flow cytometry (B) or by confocal microscopy imaging (C).