Figure 2. Rescue of lqfR null mutant phenotype by lqfRa exon 6.

(A) At left, the table shows six epitope-tagged proteins expressed in Drosophila by a UAS transgene. The columns at right show the results when each transgene was expressed in a lqfR^Δ117 or lqfR^Δ117/Df(3R)Exel6191 background with either an Actin5C-gal4 or an eyeless-gal4 driver. +: lethality and externally obvious morphological defects were rescued, − : no rescue. (B) A blot of electrophoresed adult fly protein extracts probed first with antibodies to the Myc tag (α-Myc) and reprobed with antibodies to β-tubulin (α-βtub) as a loading control. The flies contain the UAS construct indicated and an eyeless-gal4 driver. The genotypes of the flies used were: EGUF/UAS; FRT82B lqfR^Δ117/TM6B. For each UAS construct, two different P element transformant lines were tested. Note that one of the UAS-lqfRa^FL lines expressed little or no protein and this line also failed to rescue the lqfR^Δ117 mutant phenotype. The numbers at the right of the blot indicate the positions of corresponding size markers (kD). (C) Light microscope images of the eyes of adult flies. The flies are lqfR^Δ117/lqfR⁺ and their eyes are lqfR^Δ117 homozygous clones. The fly at the very left has no UAS transgene and the others contain a copy of the UAS transgene indicated, expressed by eyeless-gal4. The genotypes of the flies were: EGUF/UAS; FRT82B lqfR^Δ117/FRT 82B GMR-hid. scale bar: ∼50 µm.